Hello,

I am using IPG strip of 3-10 pH gradient for my 2-D analysis, during IEF run after attaining 1000 volt there was sudden drop in voltage. I found that there were few air bubbles formed under IPG strip at electrode wire point which were not seen at initial stages of run. Is it possible that this could be the reason of voltage drop? And if yes then how to troubleshoot this problem?

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