Pankaj Parab

5 Questions 6 Answers 0 Followers

Questions related from Pankaj Parab

Does air bubble formation under IPG strip during IEF run cause voltage drop?

Hello, I am using IPG strip of 3-10 pH gradient for my 2-D analysis, during IEF run after attaining 1000 volt there was sudden drop in voltage. I found that there were few air bubbles formed under...

08 March 2019 2,127 0 View

How to purify two different proteins with very close molecular weights and pI values?

I am trying to purify extracellular enzyme from culture supernatant, for that reason I concentrated SN and performed ion exchange chromatography using manual method (NaCl gradient in buffer,...

16 November 2018 8,390 10 View

How to perform colorimetric assay for acetyl xylan esterase?

I have gone through literature regarding colorimetric assay for AXE, but very few I could find. Some uses 4MUB derivatives and in some pNA, apart from these in one protocol bromothymol blue is...

03 May 2018 1,266 3 View

Why extracellular protein concentration decrease during late stationary or death phase in bacterial growth curve?

While carrying out growth curve of bacterial culture I was monitoring extracellular protein produced by growing biomass, I found that it decreases as my culture reaches late stationary or death phase.

16 February 2018 9,611 4 View

Hi, how to increase C18 column binding efficiency of unknown protein sample?

I'm trying to to purify protein sample using agilent HPLC system with C18 column. The moment I load sample and start program within few minutes the desired protein elute out along with solvent...

20 March 2017 6,030 9 View