Pankaj Parab

3 Questions 5 Answers 0 Followers

Questions related from Pankaj Parab

How to purify two different proteins with very close molecular weights and pI values?

I am trying to purify extracellular enzyme from culture supernatant, for that reason I concentrated SN and performed ion exchange chromatography using manual method (NaCl gradient in buffer,...

16 November 2018 8,318 10 View

How to perform colorimetric assay for acetyl xylan esterase?

I have gone through literature regarding colorimetric assay for AXE, but very few I could find. Some uses 4MUB derivatives and in some pNA, apart from these in one protocol bromothymol blue is...

03 May 2018 1,212 3 View

Hi, how to increase C18 column binding efficiency of unknown protein sample?

I'm trying to to purify protein sample using agilent HPLC system with C18 column. The moment I load sample and start program within few minutes the desired protein elute out along with solvent...

20 March 2017 5,938 9 View