Chelex vs. Glassmilk extraction approach for DNA sequencing?

Hi, I am looking into methods to extract DNA from fish tissue. Our lab has used the glassmilk approach in the past but recently switched to using Chelex. I was wondering if anyone knew of any...

24 September 2019 7,467 1 View

Is it possible to find the average of responses to a self-report scale?

My participants have rated themselves 1-10 on a series of 19 statements. Those statements are supposed to represent 5 different constructs. So for example, questions 2, 6 and 11 are all about...

06 February 2019 3,473 2 View

How long can PCR products labelled with FAM dye be stored before it begins to degrade?

I have PCR products stored at -20 degrees (Celsius) that are tagged with FAM dye. How long can these be kept before the fluorescent signal diminishes?

01 December 2018 2,081 3 View

How to calculate protein interaction energy using VMD NAMD2?

I am using the VMD plugin QwikMD to do MD simulations of my protein-protein complexes. Hereafter I am using the NAMD2 analysis to calculate my total energy of the system along with all of the...

11 May 2018 1,481 5 View

Liquid culture colony PCR technique?

I need to do a pcr on a liquid culture of E.coli. I'm amplifying a 150bp region of gyrA, using primers that I designed. I'm using NEB Next Ultra 2 Q5 MM. For the reaction, I am using 5ul NEB, 0.5...

25 October 2017 5,995 5 View

I would like to store 10 uL aliquots of bacterial culture. What would be the best way?

I need to store 10ul aliquots of bacterial culture , would the best way be to put them in PCR tubes with some gylcerol (10ul 50%) at -80?

31 January 2017 2,848 3 View

What distinguishes the fourth industrial revolution from previous radical economic development eras ?

04 March 2016 6,069 11 View

How do you prevent holes from forming in air-liquid airway epithelial cell culture after removal of apical media?

02 September 2015 9,185 1 View

Some people claim that a person can have too much antioxidants, preventing for instance the proper useful functionality of free radicals?

But maybe they are just propagandists. Might you have any information or thoughts on that?

01 January 1970 5,107 4 View

When drivers are looking at sat navs, do the distractions lead to more accidents?

Can you measure the time drivers would be looking at the sat navs and in congested urban areas would this be significant?

01 January 1970 7,179 2 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

What does Servant Leadership mean today and in the future? What are the advantages and disadvantages?

In current time of many changes in business and world of work, a lot is said and reported about the importance, skills and benefits of leadership. Are soft skills becoming more and more important?...

02 March 2021 8,149 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

What are the harmful effects of these extreme rainfall on the health of newborns? What are the reasons for that?

Extreme rainfall associated with climate change is causing harm to babies in some of the most forgotten places.

01 March 2021 5,647 4 View

What are possible instrumental variables for business dynamics (firm entry/exit)?

Dear All, For my paper, I use business dynamics (firm entry/exit rates) as an independent variable in the econometric part. Due to endogeneity I follow a 2SLS approach, for which I need a...

28 February 2021 5,322 3 View