I wants to clearly understand the role of p38 MAPK on the production of IL-10 and IL-12 or others cytokines like TNF-alpha and IL-6 by leishmania major infected DCs
No, not necessarily. Depends on receptors/other molecules involved. There are many post-receptor binding modifications happening when adding the same ligand on macrophages and DC.
Hi!
I measured for my thesis p38 MAPK activation by WB in bone marrow derived macrophages and dendritic cells in response to L. major promastigotes. In my hands, I observed that it is different. It might not necessarily be the same result if you compare other DC subsets, or if you use amastigotes for infection instead of promastigotes.
Hello,
Thank you for your response
I want to know if p38 MAPK regulates positively or negatively IL10, TNF-alpha and IL-6 production in bone marrow dendritic cells in response to Leishmania major promastigotes infection
If you have published articles on this question, can'I have a reprint of this articles
or a reprint of your thesis if possible
Thank again
best regards
Hi !
My thesis is currently under revision, but I would be happy to send you a copy once the process is completed after my defense.
Meanwhile, I would suggest you to test a p38 MAPK inhibitor in your system. If p38 MAPK activation does have a positive influence in cytokine expression, you would expect to see differences by blocking it.
Thank you very much
I'm looking forward to reading your thesis very soon
Thank you for your advice
Actually I used SB203580
Have a nice day
Best regards
Cells under stress may activate p38/JNK MAPK, however, it depends on the involvement of receptor activation. Receptor endocytosis may activate p38 or JNK depending on different structures of the parasites. Production of different cytokines depends on the recruitment of different receptors TLRs vs. others.
- Badges
- Science method