Does a Murine medulloblastoma cell line exist?

Duje Buric @Duje_Buric

02 February 2019 1 204 Report

I am looking for a murine medulloblastoma cell lines. Is there any commercially available?

Amos Bairoch

I was going to answer "no" as I currently have none in the Cellosaurus.

But then I found that there is a series of cell lines, one of which (BsB8)

seems to be quite widely used.

Here are the preliminary Cellosaurus entries for these cell lines.

ID BsB8

AC CVCL_UZ01

SY Bs-1B8

RX PubMed=10936089;

RX PubMed=11877394;

RX PubMed=22207898;

RX PubMed=30108683;

CC Transformant: NCBI_TaxID; 10632; JC polyomavirus (JCPyV).

CC Breed/subspecies: JCV T-Ag transgenic.

DI NCIt; C21993; Mouse neuroblastoma

OX NCBI_TaxID=10090; ! Mus musculus

OI CVCL_UY99 ! Bs1A

OI CVCL_UZ00 ! BsB7

OI CVCL_UZ02 ! BsB13

OI CVCL_UZ03 ! BsB14

CA Cancer cell line

ID Bs1A

AC CVCL_UY99

SY Bs-1A

RX PubMed=10936089;

RX PubMed=11877394;

CC Transformant: NCBI_TaxID; 10632; JC polyomavirus (JCPyV).

CC Breed/subspecies: JCV T-Ag transgenic.

DI NCIt; C21993; Mouse neuroblastoma

OX NCBI_TaxID=10090; ! Mus musculus

OI CVCL_UZ00 ! BsB7

OI CVCL_UZ01 ! BsB8

OI CVCL_UZ02 ! BsB13

OI CVCL_UZ03 ! BsB14

CA Cancer cell line

ID BsB7

AC CVCL_UZ00

SY Bs-1B7

RX PubMed=10936089;

CC Transformant: NCBI_TaxID; 10632; JC polyomavirus (JCPyV).

CC Breed/subspecies: JCV T-Ag transgenic.

DI NCIt; C21993; Mouse neuroblastoma

OX NCBI_TaxID=10090; ! Mus musculus

OI CVCL_UY99 ! Bs1A

OI CVCL_UZ01 ! BsB8

OI CVCL_UZ02 ! BsB13

OI CVCL_UZ03 ! BsB14

CA Cancer cell line

ID BsB13

AC CVCL_UZ02

SY Bs-1B13

RX PubMed=10936089;

RX PubMed=11877394;

CC Transformant: NCBI_TaxID; 10632; JC polyomavirus (JCPyV).

CC Breed/subspecies: JCV T-Ag transgenic.

DI NCIt; C21993; Mouse neuroblastoma

OX NCBI_TaxID=10090; ! Mus musculus

OI CVCL_UY99 ! Bs1A

OI CVCL_UZ00 ! BsB7

OI CVCL_UZ01 ! BsB8

OI CVCL_UZ03 ! BsB14

CA Cancer cell line

ID BsB14

AC CVCL_UZ03

SY Bs-1B14

RX PubMed=10936089;

RX PubMed=11877394;

CC Transformant: NCBI_TaxID; 10632; JC polyomavirus (JCPyV).

CC Breed/subspecies: JCV T-Ag transgenic.

DI NCIt; C21993; Mouse neuroblastoma

OX NCBI_TaxID=10090; ! Mus musculus

OI CVCL_UY99 ! Bs1A

OI CVCL_UZ00 ! BsB7

OI CVCL_UZ01 ! BsB8

OI CVCL_UZ02 ! BsB13

CA Cancer cell line

Badges
Science topic

More Duje Buric's questions See All

Can someone suggest a cytotoxicity test that would not include metabolic activity of the cell?

I am testing cytotoxicity of the molecules on the U251 and U87 glioblastoma cell lines. We did tests of the cytotoxicity with MTT and blue alamar, but since the molecules create radicals that...

09 October 2016 3,437 3 View

Spheroid injection into the nude mice?

Basicaly, we want to inject a spheroid as already formed 3D structure into the nude mouse subcutaneously to get tumors. Has anybody tried this? My concern is that xenograft injection requires a...

03 April 2016 253 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View