Does a .1 increment in flow cytometry scores for closely related species from 1.8 to 3.8 normally indicate an increase in the number or length of chromosomes where 1.2 to 1.4 normally indicates diploid for species in this group?
Our work with other closely related species in this genus have always shown a discrete stair step increase in flow cytometry scores when the ploidy levels as measured by chromosomes counts increase.
flow cytometry indicate an increase of ADN, but not the ploidy levels
What do you mean with a flow cytometry score? I suppose you are talking about genome sizes (C-value) expressed in pg DNA as compared to an internal standard with known genome size? Within a species genome size differences not linked to a change in chromosome number are not accepted.
I see you work on Rhododendron. Does your question relates to Rhododendron? This is a difficult species as there are many hybrids and it is often not clear to wich species group they belong. We are working on Rhododendron species as well. I will contact you on this topic in Rhododendron, if ok for you.
This question is based on research on a group of closely related wild collected species Rhododendron. Of course hybridization may be occuring naturally within this group of species.
Yes we are working with genome size 2C values produced using flow cytometry.
Please feel free to contact me.
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