Let us assume we have three species S1, S2, and S3 that are members of a group G of 100 closely related species.
Species S1 is not self fertile and has a flow cytometry score of 1.0 where flow cytometry of seeds of this species show a normal embryo / endosperm ratio of 2/3.
Species S2 is self fertile and has a flow cytometry score of 2.0 where flow cytometry of seeds of this species show a normal embryo / endosperm ratio of 2/3.
Species S3 is self fertile and has a flow cytometry score of 3.0 where flow cytometry of seeds of this species show a normal embryo / endosperm ratio of 2/3.
S1 will not easily cross with S2 or S3.
S2 and S3 will cross in both direction.
Flow cytometry of seeds of S2 X S3 show a flow cytometry score of 2.5 for the embryo and a flow cytometry score of 3.5 for the embryo for a ratio of 5 to 7.
Flow cytometry of seeds of S3 X S2 show a flow cytometry score of 2.5 for the embryo and a flow cytometry score of 4.0 for the embryo for a ratio of 5 to 8.
Root tip smashes to date are inconclusive as a means of determining the number of chromosomes or relative size of the chromosomes for S1, S2, and S3.
The flow cytometry scores for the species in Group G include 1.0, 1.2, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0. 3.1, 3.2.
The species in Group G have migrated over time from temperate to subtopical to topical and the flow cytometry scores increase along this migration path.
DNA sequencing indicates the species in Group G divide into several clades and species within these clades have similar flow cytometry scores.
Are species S1, S2, and S3 more likely to differ in number of chromosomes, length of chromosomes, something else, or no way to tell based on the information provided?
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