Is 3D cell culture considered to be a model which mimics the biological organ? Does it actually mimic the organ ?
Raktim Biswas wrote:
> Is 3D cell culture considered to be a model which mimics the biological organ?
There are two promising approaches. One is that an existing
donor organ is rid of cells so that only the scaffold if left.
It also works with xenografts. A pig heart could provide
the that scaffold for a human heart. Then the patient's
are seeded on it.
A couple of days ago a trachea was replaced by a
lab grown one with this procedure.
http://www.thelancet.com/journals/lancet/article/PIIS0140-6736%2812%2960737-5/abstract
See also
http://www.research.a-star.edu.sg/research/6456
Another method is to use 3D gels. It's difficult to get such
organs vascularized.
A new method uses sugar that is mixed with the gel.
When the sugar dissolves, it leaves channels that
may become the future blood vessels.
http://phys.org/news/2012-07-scientists-tissues-d-vascular-networks.html
Regards,
Joachim
Thank you Joachim Pimiskern, Benjamin Frey. But if I want to check any physical stress on a very small organ, then is there any possible in vitro model to study?
Dear Raktim,
> But if I want to check any physical stress on a very small organ,
> then is there any possible in vitro model to study?
I've read about a method to create artificial muscles, in this
case, to be precise, semi-artificial ones. Researchers managed
it to grow heart muscle cells on a plastic foil. Then they
could snip out stripes as they needed for experiments.
http://news.nationalgeographic.com/news/2007/09/070906-flexing-muscles.html
http://www.biologynews.net/archives/2008/04/23/heart_derived_stem_cells_develop_into_heart_muscle.html
Regards,
Joachim
Thank you very much Joachim Pimiskern ...I am going through the links provided by you...they are really helpful..
Tissue is a complex mixture of different types of cells that include as minimum endothelial cells and cells of immune system. This mixture of cell creates a unique local microenvironment. 3D cell culture of homogeneous population of cells, oh well, mimics nothing.
I mostly agree with Sergey Doronin, however cartilage is a tissue devoid of blood vessels as well as of cells different from condroblasts. Application of mechanical stress on 3D cultures of chondroblasts might possibly mimic conditions present in the original organ, although I am not an expert of chondroblast culturing. Nevertheless the effects of architectural relationships between collagen fibres and cells in cartilage would be hard to reproduce in a 3D cell culture.
Dear all,
I'm reading with a great interest all your suggestions and advices (thank you Ratkim for this topic !)nand I have a question : what do you think about fibroblasts-derived cell matrices ? This matrice was described by Beacham et Cuckierman E in 2007 (current protocol in cell biology) and several papers used this protocol to study cancer cell physiology. I'm very interesting by this matrice (rich in fibronectin) for my cancer model (leukemia) but I don't know if this matrice is really appropriate. So, what are your advices ?
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