I am trying to grow A6 (Xenopus kidney) cells on snapwell/transwell membranes but am not getting them to grow to confluence. In fact, the cells seem to peel off and die after 7-8 days. ATCC recommends NCTC-106 media + water + FBS + pen/strep. Others use L-15 media. I have also seen DMEM-F12 in the literature. Can someone help?

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