Craig R Campbell

3 Questions 7 Answers 0 Followers

Questions related from Craig R Campbell

Help with kanamycin selection in E. coli DH5a?

I have read other questions about kanamycin, but didn't answer my question. I'm trying to expand a plasmid containing an mammalian-derived Zn transporter. The plasmid also carries a...

09 September 2015 5,983 14 View

Do you use NCTC-106 or L-15 (Leibovitz) or something else for growing Xenopus kidney A6 cells?

I am trying to grow A6 (Xenopus kidney) cells on snapwell/transwell membranes but am not getting them to grow to confluence. In fact, the cells seem to peel off and die after 7-8 days. ATCC...

03 March 2014 6,565 2 View

How do you use a cell culture hood?

Perhaps a trivial procedural question, but a colleague has caused me to question what I was taught regarding sterile cell culture techniques. Primarily, they had a practice of opening the hood at...

01 January 2013 5,760 21 View