How to grow yeast BY4743 with XX::MX4 - which medium components are needed?

I want to grow yeast (BY4743) with a certain knock out (deletion), the deletion cassette is KANMX4. I assume I have to use 200mcg/ml geneticin. But can anyone explain step by step how i have to...

19 November 2020 3,434 3 View

Do you think that's nanotechnology is effective for COVID -19 therapy ?

its effective if we find the effective medication

04 October 2020 1,889 10 View

Do you support opinion that say ( there are many strains of SARS-COV2)?

read some article support that opinion

04 May 2020 5,577 3 View

How can i keep my Phenol in water solution saturated?

25 September 2019 5,766 5 View

Is there a way to eliminate non-spesific binding of HA-tagged proteins to M2 agarose?

Hi all, Im performing co-IP with Ha-tag protein lysates as prey. My problem is when i incubate M2 agarose + HA tagged protein lysate OR Ha-agarose+ flag-tagged protein lysate, my protein of...

21 January 2019 6,035 3 View

Which order is right-1 oral presentation 2 manuscript; or 1 manuscript 2 oral presentation ?

I want to present my work as oral presentation in an International congress which they will publish the summary in an International journal. If I publish this study as an full text article in a...

22 June 2018 4,518 2 View

Are there any ELISA Laboratories using SIEMENS Advia Centaur XP having troublesome Standard Deviations while measuring internal HBeAg controls?

03 January 2017 5,009 3 View

Hba1c result different between boronate affinity and HPLC method?

When comparing HBa1c analyzer in our laboratory we compared 120 patients results with each other? We performed the study in 2 same brand boranate affinity and two HPLC analyzer. All the patients...

21 March 2015 8,779 2 View

Do the dead-end streets facilitate or feed crime in their zone?

What do you think about dead-end streets? Some believe that guilty people can easily hide themselves in these streets and also they can create a closed circuit are with the help of the dead-end...

23 October 2014 3,187 15 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Is the sinovac vaccine safe and effective for people over the age of 60 ?

Dear respective researchers, I was asked by my institution to be vaccinated this week using the sinovac vaccine. However, from some of the information I got, this vaccine is not recommended for...

01 March 2021 7,781 6 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

If a given country passes mandatory 'vaccine passport rule' to go outdoors, otherwise stay at home, can it help to vaccinate all against COVID-19?

Wether vaccine passport declared not to go out of home without it may help taking COVID-19 vaccine. Can't it break individual's right to protect the community? Your comments on perspectives...

27 February 2021 5,549 23 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View