Why does the fatigue cracks initiate from surface to interior as the stress level decreases?

It is well known that low-cycle and high-cycle fatigue cracks initiate from the surface, while ultra-high cycle fatigue cracks initiate from the inside. I am very curious why this transformation...

17 January 2021 9,633 6 View

How to understand the chemo-mechanical coupling?

Recently, I came into contact with a new discipline named chemo-mechanical coupling. I found that chemo-mechanical coupling phenomena exist in many research areas, ranging from development of...

17 December 2020 1,065 3 View

I am working on membrane distillation model in COMSOL and I have some problems coming in it?

I need to add a latent heat flux of evaporation to the hot side boundary, which is a function of the pressure difference between the cold side and the hot side boundary.However, the pressure at...

16 December 2020 9,890 1 View

How to spin coat thin film for PEC devices (improve viscosity)?

I am trying to repeat the fabrication of BiVO4 (BVO) using the metal-organic deposition method. This is a well-reported way to make BVO thin film on FTO. So basically, I just dissolve Bi(NO3)3...

18 March 2020 8,405 7 View

Why ecology system allows virus exsit?

27 February 2020 5,293 3 View

Reliable PGP9.5 antibody to visualize intraepidermal nerve fibres in rat paw skin?

Hi, I'm looking for a reliable PGP9.5 antibody that can visualize INEF in rat paw skin. Most publications with good image on rat paw uses Ultraclone or AbD Serotec's rabbit anti-human antibody...

19 February 2020 979 2 View

Differential Scanning Calorimetry (DSC) protein aggregation?

I am working on Differential Scanning Calorimetry (DSC) for my protein. Mainly I want to check its melting temperature and enthalpy changes. I first tried two standard proteins ( BSA and...

17 February 2020 1,733 3 View

Why does DAB staining not work, given that TSA on the same tissue works?

I am staining for c-fos in ferret brain sections using this antibody (https://www.cellsignal.com/products/primary-antibodies/c-fos-9f6-rabbit-mab/2250). The sequence of ferret c-fos is highly...

09 January 2020 4,671 1 View

What gas flow rate should I choose for calcinating CaCO3 in the air ?

A lot of papers talk about calcination temperature, but little of them mention the gas flow rate. I find gas flow has a strong influence of my catalyst... I am preparing an organic Calcium...

18 November 2019 2,879 1 View

How to perform GC manual headspace sampling?

Hi everyone. May I know is there an established method for GC-FID headspace sampling? I did triplicates for the same concentration of analyte via manual sampling technique (using a air-tight gas...

17 July 2019 3,428 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

No title

02 March 2021 3,060 3 View