I am looking at the impact on the immune system with different treatments. Should I include the Peyer's patches when putting cells through flow cytometry? Or should they be removed and ran separately?
Dear Catherine,
You need single cell suspension for flow cytometric analysis. You can use Medimachine like tools or enzymatic procedure for single cell suspension.
Best regards,
yes, but you can get a single cell suspension from either the tissue without peyers patches or with. So its whether it skews the results too significantly?
Hi Catherine, which cells in which part(s) of the colon are you targeting and why? This is essential information; your question cannot be answered without it.
The Peyer's patches will contain distinct immune cell subsets to the rest of the small intestine, which can be further split into intraepithelial or lamina propria. The colon (after the cecum) will again contain different subsets, I'm not sure there are Peyer's patches there. So, pooling the compartments can make a difference to your results, depending on your questions.
Dear Catherine,
You can look these links, especially first one is so good.
http://www.jove.com/video/50167/isolating-immunostaining-lymphocytes-dendritic-cells-from-murine
http://www.sciencedirect.com/science/article/pii/S000887491300052X
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3910925/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3925750/
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