Do you recommend fast digest enzymes? Are they compatible with regular restriction enzymes and downstream procedures?

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Is Social Education Research a predatory or reputable journal?

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Gold nanoparticles losing binding capacity over time?

I have made these 40 nm GNPs which I purified via dialysis until no traces of contaminants were detected by UV-vis. The GNPs do not aggregate over time (lambda max does not change, neither does...

05 July 2021 8,296 6 View

Which type of pH meter and electrode should I use to measure pH of gold nanoparticle suspensions?

I tried to measure with our regular system, it took a long time to stabilize and at the end lots of gold was adsorbed on the surface of the probe to the point we had to soak the probe for days in...

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How to set calibration in SWAT+?

I'm trying to use the calibration files in SWAT+ but I have some questions on that matter. According to the Input Data documentation, several files (e.g., codes.cal, cal_parms.cal,...

15 January 2019 3,286 5 View

How do I predict next observations in Poisson sequence, using Poisson Hidden Markov Model (PHMM) in R?

I am using PHMM with R-package "HiddenMarkov" and I need to predict next observations from values of the number of customers entering a store. I hope that someone can help my with this problem

28 May 2018 1,350 3 View

How can I get the permittivity from the S parameter?

I have the S parameters of the microstrip antenna but i want to view the permitivity, the modeling software can't visualize this

18 May 2015 7,044 3 View

Call to Mexican immunologists to review evidence on "leukocyte dialyzed extract" Transferon

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How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

How combine yolo with Faster R-CNN?

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Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

Can I use Likert scale with Paired Sample T-test?

Hey researchers! I am currently doing a research about to what extent in which A has accelerated the inclusiveness of the payment system in my country. Down below are a few examples of the...

26 July 2024 5,654 3 View

What are solutions of Euler's hypergeometric diff. equation if $c- a - b$ is an integer ?

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25 July 2024 4,125 0 View

What strategy should be adopted to calculate topological indices efficiently?

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How to remove cell debris after tissue dissociation?

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22 July 2024 412 3 View

What additional processing or treatment options are available to enhance biogas plant digestate quality or value?

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20 July 2024 3,392 2 View

How does the digestate compare to other organic amendments regarding quality and performance in different countries?

Experts are welcome to share their experience country-wise so that we can make a nice document listing all the country-wise experiences. ? How does the digestate compare to other organic...

18 July 2024 5,225 3 View

Chinaza Godswill Awuchi

Kindly check https://www.thermofisher.com/us/en/home/brands/thermo-scientific/molecular-biology/thermo-scientific-restriction-modifying-enzymes/restriction-enzymes-thermo-scientific/fastdigest-thermo-scientific.html

Also check https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0090896

Aqeel yousif Alshukri

https://www.google.com/aclk?sa=l&ai=DChcSEwis0pumxrzuAhUJuncKHXA4ATUYABAAGgJlZg&ae=2&sig=AOD64_0970SfUOkhJ2RL7HTJItqCn4DuMQ&q&adurl&ved=2ahUKEwiWooumxrzuAhWxPOwKHcOJDD8Q0Qx6BAgEEAE

Triana Delfin

Thanks for the information!