Triana Delfin

2 Questions 3 Answers 0 Followers

Questions related from Triana Delfin

How to solubilized a membrane-expressed protein from a Gram+ bacteria ?

Hi everyone! I would like to lysate a Gram+ bacteria and solubilize a membrane-expressed protein. Originally, I was planning to do an SDS-PAGE gel and to perform a western blot with a specific...

25 May 2021 9,865 2 View

Do you recommend fast digest enzymes? Are they compatible with regular restriction enzymes and downstream procedures?

I have never used fast digest enzymes and I would like to know if they are effective, compatible with downstream reactions. And if any brand is better than other.

25 January 2021 4,107 4 View