Dear Camille.
The aim of your project is similar to our goals.
Our PhD. student work with Tribeč virus. Currently, we have isolated a strain of TRBV. We were curious how does it replicate in different host cell lines. During 25 serial passages in three different cell lines (VeroE6, A549 and BHK21), we have observed the presence of 3 sizes of plaques. Now, we would like to isolate them and genetically characterize. I do not have experience with plaque isolation. If you do, please, could you describe how to do it?
We use 2% carboxymethylcellulose for plaque formation test.
We would also like to do the same in tick cell line, but I did not find any in cell culture collection.
With best regards,
Tomas.
Dear Tomas,
I don't have enough skills for the moment because I began my PhD project in the last october. I will learn how to isolate viral clones from plaques with my PhD supervisor, Dr Houssam Attoui (@Houssam Attoui). I told him about your questions, you can contact him and he will answer to your questions with pleasure.
Best regards,
Camille
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