I want to detect a specific protein in a pool of exosomes from plasma by ELISA, but I don't sure if the exosomes before ELISA test are o not disrupt using RIPA buffer or another methods. What is the different? The capacity to bind at well is the same in one of this way?
If you want to detect a membrane protein you can directly perform ELISA. You can sensibilize the plate overnight with your exosomes and perform a indirect ELISA. But if you want to get a protein that are internalized in exosome you should to disrupt it.
Ok, I'll try to make both options because I'm not sure where is the protein.
Thank you André.
Hi isabel
Have you to tried to disrupt the exosomes to get their proteins ?! if so can you tell me what is the procedure you followed ?!
ThnXX
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