I'm working with exovesicles and I wonder whether exosomes can be purified using a centrifuge which reaches only 30,000 g with centrifugation about 16 hours, to obtain the same result with ultracentrifugation at 100,000 g for 1 hour.
Thanks you so much for your answer, I check the k-factor in the rotor.
Hi, I think at 30 000 g, you will have a contaminated yield of exosomes with larger microvesicles. If you are particularly looking at exosomes, it would be a good idea to filter your sample through 0.22um filter to exclude bigger MVs, in case you do not have an access to ultracentrifuge.
Hi Isabel, did you try that?? does it work?? I am really eager to hear that. is it really possible to isolate exosomes with that speed.
No, I didn't try. But I think that it isn't possible and the rotor is so important than the speep. I centrifugated in different rotor that normally I use and I didn't see the pellet and the spees was 100.000 g how the papers and protocols to isolation and purification exosomes said.
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