Do homozygous Cre-expressing mice show any problem?

hi, I am generating a loxp-Cre animal line. However, in order to keep my Cre mice colony going I want to reach the homozygous line so there will no need of PCRs. In Jackson labs they warn you...

17 May 2016 1,338 3 View

Do homozygous Cre-expressing mice show any problem?

hi, I am generating a loxp-Cre animal line. However, in order to keep my Cre mice colony going I want to reach the homozygous line so there will no need of PCRs. In Jackson labs they warn you...

04 May 2016 9,481 4 View

Does anyone know an implementation of OnPLS under R environment?

I find that STATegRa implements O2PLS and that plspm implements PLS (path modeling). I am interested in the attached application of OnPLS.

06 March 2016 2,322 2 View

Would you recomend EXOCET assay to quantify my isolated exosomes from endothelial cells?

Hi, I am looking for a way to quantify my exosomes after I have isolated them. I do not have access to a Nanosight (NTA) and although I can do EM this wont let me to quantify them. Would you...

02 March 2016 4,965 3 View

How can I calculate the iodixanol density when I am running an Optiprep gradient density for exosome isolation?

Hello, I am running an Optiprep iodixanol gradient density to isolate my exosomes. With each run I also have a control which I exact 12 fractions and from the protocol it said I need to calculate...

03 February 2016 4,172 2 View

Which housekeeping small RNA is the best for microRNA qPCR of exosomal RNA?

I think that U6 might be able to be used but I am not sure if it is secreted uniformally in exosomes. Would you recommend another housekeeping? Thanks

11 December 2015 9,133 8 View

No title

02 March 2021 324 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

What is the value of Conference Review Paper?

How much value does a review paper carry in a higher study aspirant student's profile which is presented in a Peer Reviewed Scopus Indexed conference?

27 February 2021 2,068 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View

Is it possible to remove the cells from a tissue and keep only the extracellular matrix for proteomics?

Hello! I would like to know if there is a way to flush/remove all the cells out of a tissue and keep only the extracellular matrix, in order to analyse its composition using e.g. proteomics....

14 February 2021 1,602 4 View

Where can I find the Affiliate Stigma Scale developed by Mak and Cheung?

Need the scale for a dissertation. Would be better if I could get it for free since my work is not funded. I need the English version of this scale.

09 February 2021 4,022 3 View