We collected some wild Arabidopsis plants and we were interested on the seeds on the plant and on the soil. We collected the soil surrounding the plants, which had lots of seeds on it, and we dried it. But the soil is small enough to go through the sieve, does anyone have a good protocol to separate seeds from soil?
Thank you!
Hi Carles,
The best way will be, you let them germinate and transfer them to the pots.
1. Use some different kinda sieve or filter.
2. You can try using ethanol and water in different ratios (may work but I never tried personally), make a solution, and then screen them.
Regards,
Dharmesh
To separate Arabidopsis seeds from soil, you can follow these steps:
1. Collect the soil with the seeds in a container.
2. Add water to the container and stir it gently to loosen the soil.
3. Pour the mixture through a fine mesh sieve or strainer to separate the soil from the seeds.
4. Rinse the seeds with water to remove any remaining soil particles.
5. Spread the seeds on a paper towel or plate and allow them to dry completely.
6. Once dry, you can store the seeds in a cool, dry place until you are ready to use them for planting or further experimentation.
Note: It is important to handle Arabidopsis seeds carefully as they are very small and delicate. Be gentle when separating them from the soil to avoid damaging them.
I had a fine mesh tea strainer for cleaning seeds, that worked well and was simple to use. The seeds stayed in the strainer and the dirt/dust went through the holes.
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