12 December 2014 3 996 Report

I am trying to extract genomic DNA from a small pool of mouse cells (around 60,000 cells) and the yield is critical for me (at least 80%). Does anyone have good suggestions for which genomic DNA extraction kits or protocols I can use?

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How likely DNA double strands are seperated (melted) at lower than melting temperature?

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Is there a way to reduce the interference from genomic DNA on your template-primer pairing?

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Is digestion of genomic DNA helpful to increase the pairing of primer to a specific genomic region of interest?

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