I am trying to extract genomic DNA from a small pool of mouse cells (around 60,000 cells) and the yield is critical for me (at least 80%). Does anyone have good suggestions for which genomic DNA extraction kits or protocols I can use?
Quick-gDNA™MiniPrep from Zymo Research. Quick purification of high quality DNA in less than 15 minutes using innovative Fast-Spin column technology.
Hello sir, you can use CTAB method for DNA isolation from blood cells but make sure to homogenise the sample in buffer containing beads.
Thank you guys. I noticed that a kit from Qiagen that can extract 15-25 ug of genomic DNA from 2 million Hela cells. The yield is higher than 100%.
The chimeric molecules generated during PCR are really bad for our study on recombination, since the chimeras look exactly like recombination. I wonder what and how (would be better if protocol is...
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Especially in mammalian cells.
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I am interested in all the methods or instruments used to isolate single cells especially from mammalian tissues. Any experience of you is appreciated.
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For example, the parameters for PCR are denaturing at 95, annealing at 58 and elongation at 72, while the melting temperatures of primers are around 60.
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We are trying to synthesize mouse DNA using "linear" synthesis in very limited number of cycles without "denaturing". Do you have any experience for linear DNA synthesis, especially about the the...
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For example, how many percent (if any) of DNA is melted at 5 degree lower than the melting temperature? Thanks.
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I am interested in looking for those genes that are expressed more than five fold higher when p53 is lost or mutated.
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I am trying to clean the PCR products for deep sequencing, and I would like to know which kit of which company you think is the best (based on your experience if you have done). Thanks.
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For example, whether long DNA fragments or shorter ones (such as with restriction enzymes) interfere with the pairing of the primers to the templates of interest with different efficiency or not.
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We are trying to test the efficiency of pairing of a primer to a region of mouse genome. We are thinking of digesting genomic DNA before pairing and wonder whether the longer or the shorter...
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I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
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After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
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I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
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HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
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