Yunfu Lin

12 Questions 34 Answers 0 Followers

Questions related from Yunfu Lin

What are the best ways to reduce generation of chimeric molecules during PCR?

The chimeric molecules generated during PCR are really bad for our study on recombination, since the chimeras look exactly like recombination. I wonder what and how (would be better if protocol is...

07 July 2016 9,616 5 View

What is the average disance from a mismatched base for a base substitution occurring during mismatch repair?

Especially in mammalian cells.

06 June 2016 1,376 1 View

Would you like to share with me about isolation of single cells?

I am interested in all the methods or instruments used to isolate single cells especially from mammalian tissues. Any experience of you is appreciated.

10 October 2015 466 1 View

What percent of template can be synthesized during PCR?

For example, the parameters for PCR are denaturing at 95, annealing at 58 and elongation at 72, while the melting temperatures of primers are around 60.

05 May 2015 4,418 13 View

Is there a way to reduce the interference from genomic DNA on your template-primer pairing?

For example, whether long DNA fragments or shorter ones (such as with restriction enzymes) interfere with the pairing of the primers to the templates of interest with different efficiency or not.

12 December 2014 2,385 2 View

Does anyone have experience about the efficiency for linear DNA synthesis?

We are trying to synthesize mouse DNA using "linear" synthesis in very limited number of cycles without "denaturing". Do you have any experience for linear DNA synthesis, especially about the the...

12 December 2014 5,939 0 View

How likely DNA double strands are seperated (melted) at lower than melting temperature?

For example, how many percent (if any) of DNA is melted at 5 degree lower than the melting temperature? Thanks.

12 December 2014 654 8 View

How do you think about "DNA toxicity"?

I mean the toxicity is based on DNA sequence or its structure per se not the transcription or expression products from the DNA.

12 December 2014 2,137 1 View

Is digestion of genomic DNA helpful to increase the pairing of primer to a specific genomic region of interest?

We are trying to test the efficiency of pairing of a primer to a region of mouse genome. We are thinking of digesting genomic DNA before pairing and wonder whether the longer or the shorter...

12 December 2014 6,207 2 View

Which PCR product clean-up kit you would like to recommend?

I am trying to clean the PCR products for deep sequencing, and I would like to know which kit of which company you think is the best (based on your experience if you have done). Thanks.

12 December 2014 3,043 7 View

Which human genes are strongly suppressed by wild-type p53 in human cells?

I am interested in looking for those genes that are expressed more than five fold higher when p53 is lost or mutated.

12 December 2014 9,569 2 View

Do you know of any high-yield genomic DNA extraction kit/protocol?

I am trying to extract genomic DNA from a small pool of mouse cells (around 60,000 cells) and the yield is critical for me (at least 80%). Does anyone have good suggestions for which genomic DNA...

12 December 2014 1,001 3 View