12 Questions 34 Answers 0 Followers
Questions related from Yunfu Lin
The chimeric molecules generated during PCR are really bad for our study on recombination, since the chimeras look exactly like recombination. I wonder what and how (would be better if protocol is...
07 July 2016 9,617 5 View
Especially in mammalian cells.
06 June 2016 1,377 1 View
I am interested in all the methods or instruments used to isolate single cells especially from mammalian tissues. Any experience of you is appreciated.
10 October 2015 467 1 View
For example, the parameters for PCR are denaturing at 95, annealing at 58 and elongation at 72, while the melting temperatures of primers are around 60.
05 May 2015 4,419 13 View
For example, whether long DNA fragments or shorter ones (such as with restriction enzymes) interfere with the pairing of the primers to the templates of interest with different efficiency or not.
12 December 2014 2,385 2 View
We are trying to synthesize mouse DNA using "linear" synthesis in very limited number of cycles without "denaturing". Do you have any experience for linear DNA synthesis, especially about the the...
12 December 2014 5,939 0 View
For example, how many percent (if any) of DNA is melted at 5 degree lower than the melting temperature? Thanks.
12 December 2014 655 8 View
I mean the toxicity is based on DNA sequence or its structure per se not the transcription or expression products from the DNA.
12 December 2014 2,137 1 View
We are trying to test the efficiency of pairing of a primer to a region of mouse genome. We are thinking of digesting genomic DNA before pairing and wonder whether the longer or the shorter...
12 December 2014 6,207 2 View
I am trying to clean the PCR products for deep sequencing, and I would like to know which kit of which company you think is the best (based on your experience if you have done). Thanks.
12 December 2014 3,045 7 View
I am interested in looking for those genes that are expressed more than five fold higher when p53 is lost or mutated.
12 December 2014 9,569 2 View
I am trying to extract genomic DNA from a small pool of mouse cells (around 60,000 cells) and the yield is critical for me (at least 80%). Does anyone have good suggestions for which genomic DNA...
12 December 2014 1,002 3 View