SPSS, data with 5 replicates. How to generate the variables-data sheet?

I am trying to analyze some results obtained from lipidomics. The experimental setup was as follows: 5 replicates, 3 conditions (1 control, 2 treatments). I have thus 5 numerical values per each...

31 December 2019 4,668 3 View

Can anyone suggest a robust ELISA "robot"? What would the average price be?

We are searching for an automated solution to run our ELISA assays and we would like to receive suggestions how other scientists are responding to the problem!

31 December 2018 8,008 3 View

What is the normal average level of Arachidonic acid in human blood?

I need to choose the correct ELISA assay for AA. I will be using cell culture media for the analysis, so I am prepared to obtain lower values. Nevertheless I would like to have insights on normal...

11 December 2018 4,109 1 View

What is the normal average level of Arachidonic acid in human blood?

I need to choose the correct ELISA assay for AA. I will be using cell culture media for the analysis, so I am prepared to obtain lower values. Nevertheless I would like to have insights on normal...

11 December 2018 2,257 4 View

What is the normal average level of Arachidonic acid in human blood?

I need to choose the correct ELISA assay for AA. I will be using cell culture media for the analysis, so I am prepared to obtain lower values. Nevertheless I would like to have insights on normal...

11 December 2018 1,384 2 View

Does anybody know how to prevent the biotinylation of the active recognition site of an antibody?

09 October 2014 5,370 6 View

Do you have information on alcohols as chaotropic agents?

06 July 2014 8,065 2 View

How can i get around the issues I am having with the use of GuHCl during gel electrophoresis?

I am having some issues with the use of GuHCl during gel electrophoresis, I use GuHCl to solubilize Ab peptides before loading them in SPE, and of course in the flowthrough collections I have a...

06 July 2014 8,831 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View