Do you know of any ELISA for Ceramide and/or Sphyngosine 1 phosphate?

12 December 2018 0 2K Report

Do you know of any ELISA for Ceramide and/or Sphyngosine 1 phosphate? alternatively, is there another method I can use to quantify, except for lipidomics

Badges
Science method

More Roberto Boi's questions See All

SPSS, data with 5 replicates. How to generate the variables-data sheet?

I am trying to analyze some results obtained from lipidomics. The experimental setup was as follows: 5 replicates, 3 conditions (1 control, 2 treatments). I have thus 5 numerical values per each...

31 December 2019 4,759 3 View

Can anyone suggest a robust ELISA "robot"? What would the average price be?

We are searching for an automated solution to run our ELISA assays and we would like to receive suggestions how other scientists are responding to the problem!

31 December 2018 8,075 3 View

What is the normal average level of Arachidonic acid in human blood?

I need to choose the correct ELISA assay for AA. I will be using cell culture media for the analysis, so I am prepared to obtain lower values. Nevertheless I would like to have insights on normal...

11 December 2018 2,327 4 View

What is the normal average level of Arachidonic acid in human blood?

I need to choose the correct ELISA assay for AA. I will be using cell culture media for the analysis, so I am prepared to obtain lower values. Nevertheless I would like to have insights on normal...

11 December 2018 4,184 1 View

What is the normal average level of Arachidonic acid in human blood?

I need to choose the correct ELISA assay for AA. I will be using cell culture media for the analysis, so I am prepared to obtain lower values. Nevertheless I would like to have insights on normal...

11 December 2018 1,466 2 View

Does anybody know how to prevent the biotinylation of the active recognition site of an antibody?

I have tried to biotinylate some Fab fragments but I am afraid the Biotinylation is happening also at the recognition site, thus impeding the binding of my target peptides. I would like to have...

09 October 2014 5,466 6 View

How can i get around the issues I am having with the use of GuHCl during gel electrophoresis?

I am having some issues with the use of GuHCl during gel electrophoresis, I use GuHCl to solubilize Ab peptides before loading them in SPE, and of course in the flowthrough collections I have a...

06 July 2014 8,975 3 View

Do you have information on alcohols as chaotropic agents?

I would like to avoid the use of chaotropic agents such as guanidium or UREA. Can somebody suggest to me if alcohols can serve the same purpose?

06 July 2014 8,151 2 View

What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

What do I do with dried urine samples?

I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...

14 July 2024 6,724 0 View

What can be done when electronic minimization does not converge during geometry optimization in CASTEP?

Hello All, I've been facing difficulty in executing geometry optimization of a sodium polyanionic phosphate (Na4Fe3P4O15) crystal in CASTEP (please find the input and output files attached). When...

07 July 2024 8,626 2 View

Can I directly ligate Illumina adaptor to the end of ssDNA?

I have a sequence of ssDNA that I would like to directly ligate an Illumina sequencing adaptor to. As long as I have done the end repair (remove phosphates), can I directly ligate the adaptor to...

26 June 2024 4,522 0 View

Initial and final setting times of a calcium phosphate cement?

I'm measuring the setting time of a calcium phosphate cement using an apparatus that records how much resistance a needle (connected to a load cell) encounters over time. This process provides...

24 June 2024 7,534 5 View

How do I prepare rat cardiomyocyte cell lysate for ELISA via freeze-thaw method?

Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?

23 June 2024 2,264 4 View

What is considered a biological replicate when you are doing an ELISA plate measurement with different cell types?

If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...

17 June 2024 6,705 1 View

Ni-NTA protein purification - how to obtain higher yield concentration?

Hello, I am trying to purify a 6X-His tagged protein using Ni-NTA chromatography. However, I obtain very less yield & concentration; and considerable protein in load flow through. Any...

16 June 2024 5,876 5 View