9 Questions 5 Answers 0 Followers
Questions related from Roberto Boi
I am trying to analyze some results obtained from lipidomics. The experimental setup was as follows: 5 replicates, 3 conditions (1 control, 2 treatments). I have thus 5 numerical values per each...
01 January 2020 4,735 3 View
We are searching for an automated solution to run our ELISA assays and we would like to receive suggestions how other scientists are responding to the problem!
01 January 2019 8,058 3 View
I need to choose the correct ELISA assay for AA. I will be using cell culture media for the analysis, so I am prepared to obtain lower values. Nevertheless I would like to have insights on normal...
12 December 2018 1,441 2 View
12 December 2018 4,160 1 View
12 December 2018 2,308 4 View
Do you know of any ELISA for Ceramide and/or Sphyngosine 1 phosphate? alternatively, is there another method I can use to quantify, except for lipidomics
12 December 2018 1,522 0 View
I have tried to biotinylate some Fab fragments but I am afraid the Biotinylation is happening also at the recognition site, thus impeding the binding of my target peptides. I would like to have...
10 October 2014 5,447 6 View
I would like to avoid the use of chaotropic agents such as guanidium or UREA. Can somebody suggest to me if alcohols can serve the same purpose?
07 July 2014 8,129 2 View
I am having some issues with the use of GuHCl during gel electrophoresis, I use GuHCl to solubilize Ab peptides before loading them in SPE, and of course in the flowthrough collections I have a...
07 July 2014 8,933 3 View
