SPSS, data with 5 replicates. How to generate the variables-data sheet?

I am trying to analyze some results obtained from lipidomics. The experimental setup was as follows: 5 replicates, 3 conditions (1 control, 2 treatments). I have thus 5 numerical values per each...

31 December 2019 4,668 3 View

Can anyone suggest a robust ELISA "robot"? What would the average price be?

We are searching for an automated solution to run our ELISA assays and we would like to receive suggestions how other scientists are responding to the problem!

31 December 2018 8,008 3 View

What is the normal average level of Arachidonic acid in human blood?

I need to choose the correct ELISA assay for AA. I will be using cell culture media for the analysis, so I am prepared to obtain lower values. Nevertheless I would like to have insights on normal...

11 December 2018 4,109 1 View

Do you know of any ELISA for Ceramide and/or Sphyngosine 1 phosphate?

Do you know of any ELISA for Ceramide and/or Sphyngosine 1 phosphate? alternatively, is there another method I can use to quantify, except for lipidomics

11 December 2018 1,469 0 View

What is the normal average level of Arachidonic acid in human blood?

I need to choose the correct ELISA assay for AA. I will be using cell culture media for the analysis, so I am prepared to obtain lower values. Nevertheless I would like to have insights on normal...

11 December 2018 1,384 2 View

Does anybody know how to prevent the biotinylation of the active recognition site of an antibody?

09 October 2014 5,370 6 View

Do you have information on alcohols as chaotropic agents?

06 July 2014 8,065 2 View

How can i get around the issues I am having with the use of GuHCl during gel electrophoresis?

I am having some issues with the use of GuHCl during gel electrophoresis, I use GuHCl to solubilize Ab peptides before loading them in SPE, and of course in the flowthrough collections I have a...

06 July 2014 8,831 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View