SPSS, data with 5 replicates. How to generate the variables-data sheet?

I am trying to analyze some results obtained from lipidomics. The experimental setup was as follows: 5 replicates, 3 conditions (1 control, 2 treatments). I have thus 5 numerical values per each...

31 December 2019 4,668 3 View

Can anyone suggest a robust ELISA "robot"? What would the average price be?

We are searching for an automated solution to run our ELISA assays and we would like to receive suggestions how other scientists are responding to the problem!

31 December 2018 8,008 3 View

What is the normal average level of Arachidonic acid in human blood?

I need to choose the correct ELISA assay for AA. I will be using cell culture media for the analysis, so I am prepared to obtain lower values. Nevertheless I would like to have insights on normal...

11 December 2018 4,109 1 View

What is the normal average level of Arachidonic acid in human blood?

I need to choose the correct ELISA assay for AA. I will be using cell culture media for the analysis, so I am prepared to obtain lower values. Nevertheless I would like to have insights on normal...

11 December 2018 2,257 4 View

Do you know of any ELISA for Ceramide and/or Sphyngosine 1 phosphate?

Do you know of any ELISA for Ceramide and/or Sphyngosine 1 phosphate? alternatively, is there another method I can use to quantify, except for lipidomics

11 December 2018 1,469 0 View

What is the normal average level of Arachidonic acid in human blood?

I need to choose the correct ELISA assay for AA. I will be using cell culture media for the analysis, so I am prepared to obtain lower values. Nevertheless I would like to have insights on normal...

11 December 2018 1,384 2 View

Does anybody know how to prevent the biotinylation of the active recognition site of an antibody?

09 October 2014 5,370 6 View

How can i get around the issues I am having with the use of GuHCl during gel electrophoresis?

I am having some issues with the use of GuHCl during gel electrophoresis, I use GuHCl to solubilize Ab peptides before loading them in SPE, and of course in the flowthrough collections I have a...

06 July 2014 8,831 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View