Do you have information on alcohols as chaotropic agents?

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Misha Soskine

probably not.

- alcohols precipitate proteins, even if they

are denaturing (particularly at higher temperatures)

Jeremy Jeudy

If the problem is the removal of urea and guanidin, you can consider the use of sodium deoxycholate (DOC). This chaotropic agent precipitates in acidic conditions (like 0.5% formic acid), and can be easily removed with gentle centrifugation.