The treatment of A549 tumor cell lines by IFNb induces the expression of CMH-I and also PDL1. co-culture of A549 (pretreated with IFNb) with T cells leads to partial killing of A549. I was wondering if adding anti-PDL1 could increase the kiling of A549 cells by T cells.
Thanks for guidance
From the above it's only resulted in partial killing of A549 cells which means
The co-culture of A549 cells (pre-treated with IFNβ) with T cells results in partial killing of A549 cells. However, due to the expression of PDL1 induced by IFNβ treatment, it's plausible that the interaction between PDL1 on A549 cells and PD-1 on T cells might contribute to the partial inhibition of T cell-mediated killing.
Hello Monsif Bouaboula
Yes, you are right in your thinking.
Blocking the binding of PDL1 to PD1 with either anti-PDL1 or anti-PD1 will facilitate the T cells to kill A-549 tumor cells.
The PD1/PDL1 interaction inhibits T lymphocyte proliferation, survival and effector functions such as cytotoxicity and cytokine release.
So, you should add anti-PDL1 as it would increase the killing of A-549 tumor cells by T cells.
Best.
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