Why am I getting western bands with white bubbles?

12 October 2020 6,976 3 View

Ion torrent - What happens if the amplicons are too long?

Hello everyone, What happens if I try to sequence with Iron torrent amplicons (eg of a marker gene) that are too long? By that I mean more than 500bp. We did try (not on purpose, we did not...

11 August 2020 368 3 View

Can we unbias data from umbrella simulations with missing data points?

Imagine a system with 2 energetic basins A and B separated by a high barrier. We cannot sample that barrier for whatever reason (ie. because the lowest energy path is very "long" with lots of...

11 September 2019 9,156 1 View

Can you recommend any peptide-coated microplates?

Hi! Can you recommend any company (or at least a research-team) where I can order a microplate covered with peptides according to my needs? I need it for immunological assays. Thanks for all...

12 February 2019 5,959 2 View

Can you recommend any peptide-coated microplates?

Hi! Can you recommend any company (or at least a research-team) where I can order a microplate covered with peptides according to my needs? I need it for immunological assays. Thanks for all...

01 February 2019 9,590 2 View

The nerve bundles of peripheral little nerves are twisted. This new finding is the explanation of the different reactions to acupunction?

The needle is positioned in the same nerv at different points, but the reactinons are not the sames. It can be explained only assuming that at different points of the same nerv different nervous...

27 June 2018 2,045 8 View

How to calculate Displacement at Failure in Abaqus ?

Hi I am simulating an tensile test with different speciemen geometries in Abaqus. For the Damage evolution i need the Displacement at failiure. In the Abaqus manual the displacement is defined as...

20 February 2018 7,638 4 View

Tris-Triton Lysis Buffer - any tips how to dissolve EDTA?

Hi, I want to prepare Tris-Triton lysis buffer that contains 1mM EDTA. Of course I have problems with dissolving EDTA i Tris solution (pH= 7.4). I got a tip to dissolve EDTA at pH close to 8. So,...

01 February 2018 2,955 1 View

"Clouds" in 8.8 pH Trizma-HCl buffer?

Hi, I have found my Trizma-HCl buffer (strored in a fridge) to be contaminated with sth like "clouds" (see pictures). I prepared a fresh solution and during sdjusting the pH with NaOH (40% or...

07 January 2018 9,187 3 View

"Clouds" in 8.8 pH Trizma-HCl buffer?

Hi, I have found my Trizma-HCl buffer (strored in a fridge) to be contaminated with sth like "clouds" (see pictures). I prepared a fresh solution and during sdjusting the pH with NaOH (40% or...

31 December 2017 6,330 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Apart from Nanosilica and CNT's what are the other nanomaterials readily available to use in concrete mixture?

Nanotechnology in concrete materials

01 March 2021 3,379 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View