How to convert PED and MAP files (about 600K) to Text file?

I have large PED and MAP files (about 600K) that I am trying to work with them and open them in Excel format. I opened them with EmEditor software and saved as TEXT file and then opened it in...

09 October 2019 3,849 1 View

Can I do a phylogenetic tree analysis for whole genome of my samples?

You know I have 180 samples and 8 million SNP for whole genome. Do you think I can do phylogenetic tree analysis?which method or software do you suggest for this?and how? Can anyone help me?Do you...

01 February 2016 2,088 3 View

Can anyone recommend a protocol for the extraction of oxalic acid from spinach plant?

09 October 2015 5,382 3 View

Do you think High Resolution Melt is a good technology for polymorphism identification?

I decided to amplify my desired gene sequence for some strains of my sample and after that study polymorphism but my gene sequence have a high identity to another gene so I can, t design primers...

11 December 2014 4,859 5 View

Do you think DNA/RNA used in experiments must be fresh?

Do you think after RNA extraction is better to synthesize cDNA immediately? Do you know cDNAs that were made after RNA extraction have a better quality than ones obtained from RNAs were hold in...

08 September 2014 1,619 25 View

Can anyone help me solve a problem I've been having with amplification of a specific gene by PCR?

I have a problem with amplification of a specific gene. After gene specific primer designing, I amplify my desired gene with PCR but don't get any answer. My enzyme is DreamTaq DNA polymerase. I...

08 September 2014 4,956 10 View

What kind of protocol do you suggest for RNA extraction to have an appropriate 260/230 ratio (1.8 - 2.2)?

08 September 2014 1,842 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View