Can anyone recommend a protocol for the extraction of oxalic acid from spinach plant?

More Mahboubeh Mostafaei's questions See All

How to convert PED and MAP files (about 600K) to Text file?

I have large PED and MAP files (about 600K) that I am trying to work with them and open them in Excel format. I opened them with EmEditor software and saved as TEXT file and then opened it in...

09 October 2019 3,972 1 View

Can I do a phylogenetic tree analysis for whole genome of my samples?

You know I have 180 samples and 8 million SNP for whole genome. Do you think I can do phylogenetic tree analysis?which method or software do you suggest for this?and how? Can anyone help me?Do you...

01 February 2016 2,203 3 View

Do you know about cannabinoids phytochemical expriments?

Which one do you suggest for quantitative analysis of cannabinoids: HPLC or GC? Why? What about neutral cannabinoids like THC and CBD? What about acidic form of cannabinoids like THCA and CBDA?

09 October 2015 5,659 5 View

Do you think High Resolution Melt is a good technology for polymorphism identification?

I decided to amplify my desired gene sequence for some strains of my sample and after that study polymorphism but my gene sequence have a high identity to another gene so I can, t design primers...

11 December 2014 4,969 5 View

Do you think DNA/RNA used in experiments must be fresh?

Do you think after RNA extraction is better to synthesize cDNA immediately? Do you know cDNAs that were made after RNA extraction have a better quality than ones obtained from RNAs were hold in...

08 September 2014 1,732 25 View

Can anyone help me solve a problem I've been having with amplification of a specific gene by PCR?

I have a problem with amplification of a specific gene. After gene specific primer designing, I amplify my desired gene with PCR but don't get any answer. My enzyme is DreamTaq DNA polymerase. I...

08 September 2014 5,078 10 View

What kind of protocol do you suggest for RNA extraction to have an appropriate 260/230 ratio (1.8 - 2.2)?

I need to extract RNA from cannabis. My buffer extraction is Biozol but when I use nanodrop to identify the concentration and quality of my samples, 260/230 ratio was so low. I think my samples...

08 September 2014 1,953 3 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?

31 July 2024 8,939 5 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View