How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

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23 February 2021 4,014 3 View

Build an orthogonal box in ASE?

Hello, I was wondering the best way to build an orthogonal box filled with a defined crystal structure in ASE. I know it could be easily done in LAMMPS, but I guess it is also doable in ASE. The...

21 February 2021 5,899 4 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

How to find a particular aptamer complementary sequence?

I can find several papers that give me a guide for choosing a suitable aptamer sequence 1. But how to find a property aptamer sequence 2(partially complementary to sequence 1)?

09 February 2021 3,511 1 View

Do anyone have a full MATLAB code for structural topology optimization using Genetic Algorithm? Such as a cantilever beam?

Do anyone have a full MATLAB code for structural topology optimization using Genetic Algorithm? Such as a cantilever beam? Could you provide some MATLAB code to me , a beginner for GA. Thank...

03 February 2021 3,789 5 View

How to do DNA normalization based on qPCR values?

Hi Everyone: I have a question regarding to qPCR. I am trying to do RT-PCR, and I want to have equal amount of input DNA for my RT-PCR, so I normalize my DNA input based on qPCR values of a...

31 January 2021 6,977 4 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View

Does anyone know papers that use large databases of abundance of different groups of species?

03 October 2020 2,506 4 View

Can anyone recommend a hydrophone for recording amphibian calls underwater?

I have a relatively small budget but I would like to purchase a hydrophone that would enable me to call and describe the vocalisations of frogs underwater. The animals I hope to record are in...

06 January 2020 4,280 3 View

Atypical colouration in caecilian amphibians?

I am curious to know about research publications discussing/reporting information on colour polymorphism in legless amphibians. Thanks in advance for your generous help. Venu

09 October 2019 444 0 View

Any suggestions for sample (tadpole liver) homogenization method before Ranavirus DNA extraction?

Briefly, I need a cost and time effective method - which completely exludes the possibility of contamination between samples - in order to get ranaviral DNA from the liver of EtOH stored...

17 September 2019 8,404 6 View

Any suggestions for sample (tadpole liver) homogenization method before Ranavirus DNA extraction?

Briefly, I need a cost and time effective method - which completely exludes the possibility of contamination between samples - in order to get ranaviral DNA from the liver of EtOH stored...

08 September 2019 5,526 7 View

How to quantify threats to the survival of species at Landscape Level?

I am interested to quantify threats to the survival of species at landscape level. But, I am unable to figure out the exact methods. On literature most of them just list the threats. I humbly...

04 May 2019 5,443 2 View

Hey , Any one help me? please point out the software important to analyse phylogenetic relation ship of amphibians?

I am studying Ethiopian amphibians now and I need the software to analyse the phylogenetic relation ship of amphibians.

04 May 2019 778 8 View

Checklists of terrestrial vertebrates: applying BSC or PSC?

In the last decades there is a wide use of regional, national or international checklists of terrestrial vertebrates (mainly of birds, mammals, amphibians and reptiles). Many of them use the...

01 February 2019 1,588 8 View

Can I measure molecular biomarkers on amphibians after euthanasia by liquid nitrogen?

Hi, I'm currently doing a PhD in Switzerland. Swiss legislation on the euthanasia of laboratory animals just changed. Now, euthanasia of amphibian's larvae have to be done using liquid nitrogen....

11 December 2018 4,274 2 View