yes specially in Mid- Iraq but the F, gigintica are higher prevalence

Dear Zhaily González Rodríguez

It depend on endemicity

Here in Sudan it is rare

Dear Zhaily

In 35 years, I have only found Fasciola hepatica in bilis samples just twice. These two patients had eaten contaminated water-cress in San Luis, an endemic region in Argentina .The diagnosis was based on a simple sedimentation technique and microscopic examination.

Thank you very much for your answers.

Invasion of human with Fasciola hepatica in Russia is extremely rare, opisthorchosis are more common. The diagnosis is based only on the study of stool by ether-formalin sedimentation. A significant number of false positive results are recorded using the enzyme immunoassay

In Madagascar, apart from schistosomes, trematodes are extremely rare. in my studies, only nematodes were found in greater numbers.the standard parasitological methods (Kato-Katz and merthiolate-iodine-simple formol( MIFs)) were used

Dear Zhaily

Fasciola hepatica infection is an accidental event. In order to find it in Cuba you have to take into account several factors including environmental and epidemiological conditions. You can find it more frequent in bovines. I remember when we were residents we saw sporadic cases of this infection in humans. Fortunately, the Institute of Tropical Medicine Pedro Kouri is in charge of the national surveillance of this parasite in humans samples. Thank you very much for your work at this institute.

Fasciola hepatica eggs in Calabar area of Nigeria was regularly found in stool samples in the 1980s but very rarely seen since the 1990s to date using the Formol- ether concentration technique. I am worried about using the immunological technique since helminths share heterogenic antigens.