Wanted to know the normal range for serum endothelial nitric oxide synthase assay and acceptable values for patients with CKD and cardiovascular disease. Thank You
A new cell subtype was found out only during regeneration phases after a cuprizone-induced demyelination model. How can I characterize this cell? It is important to highlight that was already...
13 February 2021 1,245 1 View
Dear researchers, I am coupling Design Optimization with ANSYS Fluent in Workbench 2020 R2. I can do the analysis. I am patching the initial Temperature values of the zones. However, the patch...
28 January 2021 4,265 2 View
To perform the Luciferase assay for microRNA-gene interaction its necessary a primer (wild-type) to amplificate the specific ligation site and also a mutated primer. But if your primer has a...
19 January 2021 8,568 3 View
Hello, I would like to use the software Moldex 3D for the design optimization of an injection mould. How can I couple Moldex 3D with a MATLAB code? Any help appreciated, I wish you all a happy...
09 January 2021 569 2 View
Hello, I am using ANSYS Fluent for simulating the filling stage of an injection mould. However, when comparing the results with those of specialized software, such as Moldex 3D, I found the...
07 January 2021 7,992 1 View
In recent years our group has highlighted the importance of trees and plants in public and recreation parks in large mega cities. However, we are interested in developing "green shields" to be...
05 January 2021 6,500 3 View
Hi everyone, I am using the pyTMs plug in on Pymol to phosphorylate a particular threonine in my protein. I am having difficulty in selecting only one or two residues - it seems I can only...
05 January 2021 230 3 View
I would like to receive help, as I am trying to make an analysis of the ethanol PES, but the calculations are always giving error. I use the conda-forge version of xtb (* xtb version 6.3.2...
04 January 2021 602 2 View
Hi everyone! I am trying to use STATA to create a funnel plot to asses publication bias using proportions from studies for a large meta-analysis (having used the metaprop command). I"m having...
09 December 2020 6,096 3 View
I need to include the bending mode dynamics in my simulation. So far, I am only considering the rigid body dynamics. So I need to implement the following equation: q'' + Kg*q = Phi*F(t), where:...
19 November 2020 4,270 5 View
We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...
02 March 2021 6,036 1 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...
28 February 2021 7,310 3 View
I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.
28 February 2021 6,512 3 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...
26 February 2021 928 6 View