I want to enhance soprulation of an isolate that was identified by Vitek2 and found to be Bacillus cereus/mycoides/thuringiensis.
Can I add traces of certain ions to TSA media to enhance this sporulation process in short time (3 days)?
Hi Mohammed
Add MnSO4 (5 mg/L) to TSA in order to enhance sporulation, you should see spores in less than 3 days.
Hope this helps
Dear MMA-E,
CaCl2.2H2O, MgSO4.7H2O, and MnSO4.H2O have been found to be helpful in sporulation. Especially, CaCl2.
Please follow this article, I hope the same can be used with agar medium as well.
http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12548/full
Let me know if you need PDF of any of other works of our group.
Regards
Dr. Prasun
Dear Mohammed,
I would also suggest the AK agar medium, which is a sporulating medium. I used it with B. thuringiensis with good results.
From BD:
http://catalog.bd.com/nexus-ecat/getProductDetail?productId=210912&parentCategory=1211&parentCategoryName=Microbiology%20Testing&categoryId=1269&categoryName=Dehydrated%20Culture%20Media&searchUrl=
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