How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

What is the maximum temperature the soil can reach in a ferruginous rock field?

I searched online but I could not find any answer to it. Does anyone ever measures it or knows a work on this topic with that info? Thanks :)

10 July 2019 1,415 3 View

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23 July 2017 3,340 4 View

Is it necessary that there is a T7 terminator at the end of an insert in a plasmid for the transcription of a sequence?

I would like some help with the following topic: I have a plasmid with my sequence of interest which is a chimeric virus. For the transcription I inserted a T7 promoter site before my insert....

12 December 2016 4,545 3 View

Can anyone suggest a reliable loading control for rat skeletal muscle samples?

28 April 2016 4,343 6 View

I am having a problem keeping 3T3-L1 cells alive when differentiating them with troglitazone. Has anyone encountered this?

I grow 3T3-L1 cells for my research. In the differentiation process, I use a medium that contains troglitazone for 2 days. This has worked very well for about 1year, but the past couple of time,...

26 May 2015 8,101 5 View

Does anyone know an alternative to ether + creosote as an antifungal cleaner for drying insects?

I've already heard that (white) merthiolate could be used for this purpose...

08 September 2013 466 1 View

The genus Anopheles exists only among insects. the use of "Anopheles mosquito" is rampant. Why not simply say Anopheles or mosquito? please educate me

01 January 1970 2,762 13 View

Which applications do you consider important for the health area?

Which applications do you consider important for the health area?

01 January 1970 3,273 11 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Difficulty in debugging code to correct error - TypeError: '<' not supported ?

I am trying with a Unet code ontained from net ...in tensorflow. When trying to predict the segmentation result with the trained and saved model with metric " iou" using "CustomObjectScope"...

22 February 2021 5,254 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View