I purchased the cell line LL29 from the ATCC a while ago. This cell line is isolated from the fibrotic lung of the IPF patients. I believe this cell line is already lung fibrosis disease cell line.

I have just one doubt to clear.

Do I need to add TGF-beta into this cell line to promote EMT?

I believe TGF-beta is not needed here because it is already IPF disease cell line and it contains ECM proteins upregulated.

Please give some insights.

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