I purchased the cell line LL29 from the ATCC a while ago. This cell line is isolated from the fibrotic lung of the IPF patients. I believe this cell line is already lung fibrosis disease cell line.
I have just one doubt to clear.
Do I need to add TGF-beta into this cell line to promote EMT?
I believe TGF-beta is not needed here because it is already IPF disease cell line and it contains ECM proteins upregulated.
Please give some insights.