Dear Mr.Jan Arends

Thank you very much for your help.but this paper is not freely available.

If you have, can you help me second time by providing me this paper?

Dear Jan B. A. Arends

Thank you very much for your kind support

The fresh or non-inoculated cooked meat medium doesn't need to add oil. But if you want to store some anaerobic isolates, it'd be better to add sterile mineral oil on the surface of the culture.

I used to maintain some clostridial isolates the mentioned way for at least 3 months with cooked meat medium without sugar, in room temperature.