Do preadipocytes and stem cells express inflammatory cytokines during differentiation in cultured conditions?

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Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

How to increase citation in Research Gate?

How to enhanced h-index in Research Gate?

04 August 2024 3,368 4 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

What is meant by baseline of FTIR data?

I got comment on my FTIR data figure from a reviewer. The reviewer said "FTIR data in Figure should be repeated. there is no bassline." I made Y off set comparison graph of FTIR on OriginLab. Can...

03 August 2024 6,070 3 View

What is Random Audit?

HI there, I've came across several articles discuss about random audit an Non random to tax evasion or compliance. Most of the articles is relating about effect of audit (random or non random)...

31 July 2024 5,309 7 View

Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...

31 July 2024 9,697 0 View

How to do wavelet transform of EXAFS data?

I want to do wavelet transform of some compounds for their comparative study? Kindly help me in this regard and provide with proper procedure.

31 July 2024 4,170 0 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

How to use energy flexibility in inventory modeling?

29 July 2024 2,192 5 View

Can we use technical replicates for statistical analysis of multiplex cytokine data?

I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....

31 July 2024 3,665 3 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

We are not able to successully reproduce 3t3-L1 cell differentiation, what could be the reasons?

Greetings all, My group has been trying to reproduce 3t3-L1 cell (preadipocytes) differentiation. The typical protocol involves 3 steps: the first step and second step both last for 2 days, and...

18 June 2024 2,728 1 View

How to image faint cells on microscope (Serum Free Media)?

I have a peculiar problem - in FBS or albumin containing media my cells (preadipocytes) are very easy to image (and therefore quantify using a combination of ilastik and CellProfiler). However,...

28 May 2024 3,780 0 View

Mouse MSCs not expressing TSG-6 on cytokine stimulation?

I am using mouse bone marrow derived MSCs (passgae 3-6) for my in vitro cytokine priming experiment. My basal media is 2% FBS-DMEM. I am using 10 ng/ml TNF-a, or IFN-g or both for 24 hours. I see...

27 May 2024 8,935 0 View

3T3-L1 cells not differentiating ?

Hi, I have been working with 3T3-L1 cells for over a year now.However, over the last couple of months they stopped differentiating into adipocytes. I seed at 200k cells/well (12 well plate), and...

23 May 2024 3,356 1 View

BD CBA mouse Th cytokine kit vs. Biolegend mouse Th LEGENDplex, which one has better performance?

Hi, Just a quick question for those who have profiled cytokines using sera or cell culturing media supernatant samples. I compared the sensitivity of these two in their manuals and found BD CBA...

14 May 2024 4,596 0 View

How do I get the mathematical model for a flow cytometry assay?

Hi, everyone. I'm tasked with mathematical modeling intracellular cytokines using flow cytometry. Does anyone have a suggestion for the approach I should follow? Maybe there is a technical note...

28 April 2024 4,340 0 View

Does anyone have recommendations which dilution is fine while measuring serum Ang 1, Ang2 and VCAM-1 levels?

Dear colleagues, I will measure serum Ang 1, Ang 2 and VCAM-1 levels by ELISA in patients who were undergone hematopoietic stem cell transplantation. I should find the most suitable dilutions...

21 April 2024 5,222 1 View

How can intramuscular preadipocyte be isolated from rabbits?

Please i need a proper protocol to isolate intramuscular preadipocyte from the rabbit

16 April 2024 9,395 0 View