What are the subunits of rRNA (in the larger units or 60s) do plants have?
https://en.wikipedia.org/wiki/Ribosomal_RNA#Subunits_and_associated_ribosomal_RNA
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
We are working on a robot that cleans solar panels using fresh water supply and a rotating brush. We are trying to conserve as much water at possible by recycling the dirty water that is collected...
28 July 2024 5,778 2 View
Is it the best optimization ratio based on experiments?
22 July 2024 8,170 0 View
Can diamond be grown using molecular beam epitaxy?
22 July 2024 9,755 2 View
Hello everyone, I am new to LabVIEW, right now we need to control the movement of an ethernet-based stage drive. We got the sample code from a collaborator but their instrument is USB-based, so we...
11 July 2024 5,385 2 View
Hi everybody, We are trying to write a systematic review meta-analysis paper. But I could find 19 references. I think 19 references are not enough to do a meta-analysis section and it is better to...
10 July 2024 5,490 5 View
I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.
01 July 2024 8,361 3 View
I have my RNA samples eluted in nuclease-free water. But now I want to store it for a longer period. Can I add isopropanol directly to my samples to store it without doing a second round of TriZol...
23 June 2024 2,576 4 View
I'm simulating a metamaterial unit cell. I want to plot Frequency Vs Phase Response. Also I want to extract permeability and permittivity. Kindly share the steps.
23 June 2024 3,446 1 View
Hi, I am very new to Bioinformatics. Recently, I have the project that aims to perform taxonomic anlysis on raw reads from mixed samples taken from environment. For example, we perfromed NGS...
09 June 2024 6,537 3 View
Hi all, I have just started to learn about bioinformatics and I need help with it. I have enriched some microbes from wastewater anaerobic sludge and sent them for 16S rRNA sequencing. Based...
29 March 2024 5,278 4 View
Do I need to repeat the process or can I go for morphological/ Biochemical tests for fungi identification?
21 March 2024 5,153 1 View
I'm running RNA extracted from Saccharomyces cerevisiae in a Tapestation 4200. The RINe value is excellent, but the sizes of the 18S/28S bands are lower than expected: ~1000 and ~1800 instead of...
03 March 2024 7,681 1 View
Having run and analyzed a few thousand RNA samples on the Fragment Analyzer I have, in rare occasion, observed samples with no 28S peak in perfectly intact RNA. The samples are not from insects or...
24 January 2024 4,083 0 View
Hello everyone! I am working on my undergraduate thesis. After I used the DNeasy® PowerSoil® Pro Kit to extract DNA from a coral mucus sample I use 2 microliters of DNA to measure the output's...
15 December 2023 4,751 1 View
Hi all, I have been testing housekeeping gene stability in human dermal fibroblasts, ipsc and ipsc-derived cells for circadian studies. Using the same set of primers, the Ct for 18S rRNA is about...
22 November 2023 3,742 1 View
SRR no. input filtered denoisedF denoisedR merged Nonchim % drop SRR15372855 210711 186522 175583 176162 116563 36509 83% SRR15372856 209697 183184 173837 174328 127588 38205 82% SRR15372857...
21 October 2023 9,061 3 View
Hi, I am currently trying to isolate RNA from whole blood. I have frozen blood samples - preserved in RNAlater stored at - 80. Samples were collected in RNA later (300 ul blood sample and 1.3 ml...
12 October 2023 267 3 View
I am using universal 16S rRNA primers to screen samples of genomic fungal DNA for the presence of bacterial DNA. The resulting band should be 1460bps, but there are lots of other bands of larger...
09 October 2023 6,997 0 View