Is loss of 80% sequences after chimera removal in 16s rRNA preprocessing normal? if not what should I do?

More Saesha Verma's questions See All

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

How to estimate the extent to which the FE domain should be meshed with explicit elements in Explicit-Implicit Time integration procedure?

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How to adjust a covariate in meta-analysis?

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11 January 2021 9,409 4 View

Is it possible that first waiting time decreases and then after a certain point waiting time start increasing?

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22 December 2020 2,455 1 View

Which gas is used for Bunsen burner in ASTM D6413 Vertical Flame Test?

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09 November 2020 1,808 1 View

How does culture impact our ability to learn?

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14 October 2020 9,107 7 View

How to produce ROC/AUC curve using various groundwater vulnerability method for comparative analysis?

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When to add H2DCFDA dye before or after treatment and why?

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Poisson's ratio of nano hydroxyapatite?

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30 June 2020 7,704 5 View

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27 April 2020 6,536 13 View

What are the values needed for gabor filter's parameter for cifar10 data?

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How to calculate Total Flavonoid?

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RNA-seq DEGs downstream analysis and reporting in literature?

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23 February 2021 8,969 1 View

What are the ranges of four diffterent poles of butterworth filter for ECG signal?

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Which polarizers are usually used to measure the birefringence of zebrafish muscles?

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What low-cut filter (high Pass frequency) to be used for response spectrum (Tripartite plots) ?

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Is it possible, for a limited dateset of images, to use Convolution filters + Random Forest instead of UNet?

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The type of filter paper used to filter an epoxidized oil (the mixture is an epoxidized oil with magnesium sulfate) ?

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Why ICP-MS test for Teflon filters that collected PM2.5, show really high results in the millions of ug/g ?

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Timur Yergaliyev

Hello!

Did you remove primers with cutadapt before dada2? Primers in sequence may cause high false positives among detected chimeras.

Here is the link to the qiime2 forum: https://forum.qiime2.org/

Saesha Verma

Thank you Timur Yergaliyev

It didn't have adapter contamination. I did this filtering in the R using DADA2 package.

Aia Oz

It's not standard. If you didn't resolve it yet, try using the 'pseudo' option in dada2.