I have a lyophilized fluorescent peptide that should form alpha helical structure in theory. The sequence is as such:FITC-(EIAALEK)3. Will those 21 residues form alpha helix as soon as I resuspend the peptide in water?
Yes, they will, as long as the pH is right and the concentration is high enough for the expected Kd. If I remember well those designed coiled-coiled should in the high nM to low µM, and the kinetics is not super fast, so you might want to give it a few min to make sure they reach equilibrium. We have a relatively recent paper on those systems that might be useful for you.
E. Pazos, A. Jiménez, J. L. Mascareñas and M. E. Vazquez, Chem. Sci., 2011, 2, 1984–1987. If you're interested it's available in my profile.
Yes, they will, as long as the pH is right and the concentration is high enough for the expected Kd. If I remember well those designed coiled-coiled should in the high nM to low µM, and the kinetics is not super fast, so you might want to give it a few min to make sure they reach equilibrium. We have a relatively recent paper on those systems that might be useful for you.
E. Pazos, A. Jiménez, J. L. Mascareñas and M. E. Vazquez, Chem. Sci., 2011, 2, 1984–1987. If you're interested it's available in my profile.
Yes, at particular pH, temperature, ionic cloud petides change their topological shape spontaneously.
Dear Zeynep Hein ,
Yah, Sure The peptide will form secondary structure spontaneously, due to it's environment that you have created that means, at particular temperature, pH. so for your condition it's sure no doubt. i agree both answer above too.
Good Luck & Regards
R.Selva...
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