Zeynep Hein

4 Questions 13 Answers 0 Followers

Questions related from Zeynep Hein

Do peptides spontaneously form secondary structures?

I have a lyophilized fluorescent peptide that should form alpha helical structure in theory. The sequence is as such:FITC-(EIAALEK)3. Will those 21 residues form alpha helix as soon as I resuspend...

04 April 2015 2,880 4 View

Can anyone suggest a cell surface protein endogenously expressed in Vero cells that I can stain in immunofluorescence?

I would like to know if anyone can suggest a cell surface protein endogenously expressed in Vero cells that I can stain in immunofluorescence without having to permeabilize the cells. So the...

10 October 2014 2,252 4 View

Can I use ECF subsrate in ELISA?

I wonder if it is possible to use ECF substrate which fluoresces when alkaline phosphatase is present. We have a fluorimeter (plate reader) so I thought of using our ECf to develop an ELISA...

09 September 2013 9,471 1 View

Can a cracked gel be saved (SDS-PA)?

My 11% sds-pa gel broke into very many pieces during drying when the pump suddenly stopped working all of a sudden. is there any way to reconstitute the gel? It doesn't need to be pretty I just...

10 October 2012 3,910 19 View