I have just started culturing MRC-5's and noticed that I usually get half the expected number of cells (compared to other cell lines that I have cultured) at confluence. For example, 4 million cells in a T-75 and 9-10 million in a T-175. Is that typical for this cell line? I have noticed that they are visually larger than most cells that I have cultured previously.
Suhad Mohammed
https://www.researchgate.net/post/Any_suggestion_for_improving_MRC-5_cell_line_growth_rate
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Lady López
Indeed! I work with MRC-5 cells and 5 million cells is what you are actually going to get all time when they are in early passages, and that's at maximum confluence (in a T75 flask) As they grow old, they start growing less, and it takes more days to get a fitting number (2-3 million)
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