Has anyone succeeded in capturing uranium atoms in a fullerene structure?

Additional questions are: What are the encapsulating atoms? How difficult was the process and what rates were achieved? What factors most seriously affect the economics?

20 February 2021 3,134 5 View

Does anyone know a company that does Pyrosequencing to determine mitochondrial DNA mutation heteroplasmy?

We are looking to genotype our mice. Our mice have a mutation in their mitochondrial DNA and we want to know the level of heteroplasmy i.e. % of mutation in their mitochondrial DNA. So we cannot...

09 November 2020 4,246 3 View

Low yield with HCl in chalcone synthesis: Why?

Hello, I can't understand why a low yield is obtained with the use of HCl in aldol condensation in acid catalysis for the synthesis of hydroxychalcone.

28 July 2020 7,853 5 View

Why do I get the same model fit indices for bollen-stine bootstrap and ML in AMOS?

Hi Guys, I would highly appreciate some help/input regarding boolen-stine bootstraping in AMOS. Given that my data violated the multivariate normality assumption I have opted to run bollen-stine...

12 May 2020 2,391 3 View

Positive Zeta Potential NPs dispersed in water?

Hello guys I am looking for positive zeta potential colloidal particles dispersed in water which I can order online. pH around 6 to 7. The size its from 300 nm until 700 nm. Thanks a lot for...

28 March 2020 6,869 3 View

Do you have weekly records of most read in dept that we can access?

I would like to know how many weeks my article, Review of School Climate Research, has been the most read in my department , Psychology, Fordham University, Thanks for your help, Ann...

16 September 2019 3,313 3 View

Does anyone have experience with antibodies from Invitrogen (Flow Cytometry)?

28 April 2019 2,718 2 View

Low signal - Circadian luciferase assay in cell culture?

Hi, we are running circadian luciferase assays in cell lines (u2os and 3t3) driven by two different circadian promoters. We're reading the assay in a TECAN m200 luminometer using a black clear...

01 November 2018 7,642 1 View

Less Primitive Way to Determine Maximum and Minimum Node Number in Component in ANSYS?

Hello, I would like to determine the node numbers which make up one of the parts in my geometry in ANSYS. The way that I am currently doing this (which works, but is inefficient) is to view the...

16 July 2018 8,935 3 View

How to Apply Voltage to Piezoelectric Stack Actuator?

Hi, I have what I think is a simple question that I cannot find the solution to. I am modelling a piezoelectric actuator with a stack system (PZ disc, electrode, PZ disc, electrode, etc.), but I...

19 June 2018 9,653 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View