Please suggest me whether MCF-7 cancer cell produce caspase-3 during apoptotic cell death?
There is quite a body of literature showing that MCF-7 cells do not express caspase-3 at all. As far as a I know, caspase-3 independent apoptosis pathway has been studied in MCF-7 cells. So the short anwser to your question would be: no.
Googling "MCF-7 caspase-3" will provide plenty of information on this issue.
Hello
Regarding MCF-7 cells they are deficient or absent of caspase-3, however they still undergo apoptosis through intrinsic apoptotic pathway so they release cytochromme c and activate caspase-9 go for sequential activation of caspase-7,6 and 8. also the function of caspase-7 is similar to caspase-3 any way.
That's correct. The cells are capase 3 deficient. As for caspase-3 independent cell death, see the paper linked.
Article Macrophage-mediated clearance of cells undergoing caspase-3-...
I agree with the above answers. You can not read more about MCF-7 cells and caspase 3
As shown in the below picture, the apoptosis signaling pathway is much more complicated than expected. In parallel with cleaved caspase-3, the cascade of activation of a caspase-9/Apaf1/cytochrome c apoptosome should be evaluated when MCF7 cells undergo apoptosis. Besides, caspase3 activation leads to the cleavage of caspase8, which may also contribute to cytochrome c-mediated cell death.
MCF7 cells don't express caspase-3, so if you are trying to identify caspase-3 cleavage products by western blotting that is not going to work.
If you are doing activity assay (with DEVD-AMC for example), you are probably detecting the activity of caspase-7, which is expressed in MCF7 cells and which exhibits a certain degree of functional redundancy with caspase-3.
My previous lab mate Dr. Eun Young Choi, has published on caspase-3 and caspase-7 (see link bellow) and I would recommend her article, in which she refers to many seminal works in the field. Hope this helps.
http://www.jbc.org/content/284/19/12772.long
But there are MCF-7 that exogenously express caspase-3 GFP. They are published an available
MCF7 celos are caspase 3 deficient but apoptosis is posible in absence of caspase 3, by activación of caspase 7 or 6.
I thank each and everyone who had shined the light on my knowledge. Actually I am evaluating a drug molecule for cancer activity. Inorder to study intrinsic and extrinsic pathway, we used high content screening method. During our analysis, we found caspase 3 is activated both in intrinsic and extrinsic pathway. However, I see many reports indicating caspase 3 is not activated in MCF-7 cells. I am bit confused.
I also have observed upregulation of Caspase 3 in MCF-7 cells after treatment with some drug-loaded niosomes.
The reason is not clear but I am sure about the data.
I am reached to the same conclusion that there is upregulated Caspase 3 in MCF-& after treatment with drugs loade with sulfonamide