Sabarinathan Rangasamy

7 Questions 9 Answers 0 Followers

Questions related from Sabarinathan Rangasamy

Why does a macrocyclic compound which can be separated by a normal column (manually) was not able to separate or detect any peak by using HPLC?

I tried to purify a highly water soluble compound using RP-HPLC. Unfortunately, no UV peak was detected, however, when a same compound was run on a normal C18 column, there were three different...

17 June 2015 4,961 5 View

How can I calculate singlet oxygen quantum yield of a photosensitizer by using the phosphorescence method?

Please provide me a step by step protocol to measure the quantum yield.

06 May 2015 5,518 1 View

Do MCF-7 cancer cells activate caspase-3 during apoptosis?

Please suggest me whether MCF-7 cancer cell produce caspase-3 during apoptotic cell death?

30 January 2015 4,009 12 View

How do you detect singlet oxygen and calculate its quantum yield by phosphorescence?

I wanted to measure the quantum yield of singlet oxygen by phosphorescence. In this regard my questions are: 1. How much concentration is required? 2. Do I need to measure both in aerated and...

20 November 2014 3,071 6 View

How to purify a sulfonated porphyrins or the other organic compounds?

I have prepared sulfonated porphyrins and purified by soxhlet extraction. But MALDI-TOF analysis showed other impurities. I would appreciate if you could suggest me some suitable method to purify...

17 September 2014 9,544 5 View

Is chloroform suitable for cell culture experiments?

Please suggest me whether minimal amount of chloroform (0.1%) is suitable to use in cell culture experiments. As my organic compound shows degradation behavior in DMSO solvents, I prefer using an...

21 August 2014 9,611 13 View

How can I measure Laser power using a power meter?

I wanted to measure Laser power for using it in a biological application. I can understand that as the distance of the laser is adjusted, the beam/spot size is also varied and subsequently power...

14 August 2014 4,027 5 View