I want to construct a lentiviral plasmid containing Alu elements. So, do Alu elements have any influence on lentivirus integration. Will thet be recognized by integrase(IN) influencing the insertion ?
Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...
05 August 2024 5,829 1 View
I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...
22 July 2024 5,790 1 View
science teachers' professional vision:framewor,evaluation,review of related research,researchers related,research method,why professional vision
20 July 2024 6,577 1 View
In dentistry, we sometimes need ‘binocular diplopia’ when dealing with complex dental problems, which means using different angles and tools to ensure the treatment is effective. In your opinion,...
09 July 2024 3,898 0 View
I synthesize silver nanoparticles ,and reserve it in water. Then, when I sonicate it some minutes or longer ,it couldn't be dispersed, most of them aggregate .Otherwise ,when I sonicate several...
09 July 2024 7,335 0 View
This research question explores the complex interactions between oral microbes and the oral sensory system. The diversity and function of oral microbial communities play an important role in oral...
09 July 2024 3,601 0 View
Recently I've been working on a simulation of concrete 3D printing, and has successfully simulated a layer-by-layer 3D printing process using model change. However, when I proceeded to try...
06 July 2024 2,130 0 View
The material is SiGe
03 July 2024 1,374 0 View
I already used SiliconGermanium.par why it still error someone can explain this thank you!!
27 June 2024 9,269 2 View
I currently need to purify DNA after plasmid linearization. I am using the TaKaRa DNA purification kit, but the concentration of the diluent obtained from each DNA purification is very low. Do you...
23 June 2024 456 3 View
I want to know more about REE deposits.
03 August 2024 6,930 2 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
31 July 2024 7,366 1 View
Hello, I am a master's student currently conducting lentivirus transduction experiments in Jurkat cells. The virus is not effectively entering the Jurkat cells, so I am planning to use an...
21 July 2024 4,676 4 View
i need to ask that when I transduce my HEK293T cells with Lentivirus, how much time will they require for transduction because when I add lentivirus and then change the media after 24 hours, my...
07 July 2024 2,946 1 View
01 July 2024 6,680 2 View
We aim to study an exothermic process and evaluate its temporal changes. Could you recommend suitable software for this purpose and explain how to conduct this analysis? What specific data is...
30 June 2024 8,413 1 View
Hello researchers, i need some help, i am packaging Lentivirus( having my transgene) in HEK293T cells, as I will collect the supernatant and concentrate my lentivirus,I also want to check...
15 June 2024 1,596 3 View
I'm attempting to develop a method for analyzing rare earth elements by ICP OES, and am hoping to find an internal standard to use for the analysis. I've tried several, and they simply do not...
09 June 2024 773 6 View
Hi there, I have transfected these two plasmids into 293T packaging cells to encapsulate a pLVX-M-Puro coding plasmid. I had already validated the stability of the pLVX-M-Puro insert by transient...
06 June 2024 8,274 5 View