Yuxin Liu

4 Questions 1 Answers 0 Followers

Questions related from Yuxin Liu

How strong the interaction between target RNA and hybridized probes is?

During RNA FISH, the fluorescent probes are hybridized with target RNAs with 20-24bp base pairing. But how strong this interaction is with only several hydogen bonds? How much salt or fomamide...

15 December 2023 8,853 3 View

Do internal complementary sequences influence the integration of lentivirus into genome ?

I want to construct a lentiviral plasmid containing Alu elements. So, do Alu elements have any influence on lentivirus integration. Will thet be recognized by integrase(IN) influencing the insertion ?

19 December 2020 2,381 0 View

Again, what are the bands of extracted plasmid?

I used Trans1-T1 phage resistant chemically competent cells to extracted lentiviral gRNA expression vectors. And there were bands near 2000bp and smear on 1% argrose gel. Did the smear come from...

26 November 2020 1,149 2 View

What are the bands of extracted plasmid?

I used MN NucleoBond Xtra Midi to extract plasmids, but the agarose gel electrophoresis showed the plasmids contained not only one component. There are bands around 250bp, what are those bands?

20 November 2020 2,464 3 View