Do I need to remove microbeads before putting CD31+ positively selected cells in culture?

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Why non-technical jobs are more paid at the beginning???

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Do you think your physical fitness can impact your research capabilities?

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Could anyone help with why the protein (Myc-DDK tagged) not expressing in HEK293T cells?

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Is there any software for predicting disulphide bonds in a protein?

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What are the best possible softwares for ORF prediction?

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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fatal error

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fatal error

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fatal error

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fatal error

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How to repeat an operation on an atom selection for multiple times in PyMol?

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New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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John Schloendorn

There are manufactures that claim their beads are internalized and biodegraded completely. Indeed, we found that cells isolated with iron beads from Miltenyi lost their magneticism after some days in culture (but it could be due to dilution). We didn't see any particular unexplained results with bead-isolated cells.

It's supposed to be possible to remove the beads by cleaving the antibodies with papayin or DTT. We didn't try that. It would allow you to compare head-to-head.