Is anybody in Sweden interested for collaborative Indo-Sweden Research Project?

Warm Greetings! I'm glad to bring to all of your notice that there is a call for collaborative research project funding from the Department of Science and Technology, India entitled "INDIA-SWEDEN...

04 August 2019 2,049 8 View

No title

23 May 2019 3,584 10 View

Why non-technical jobs are more paid at the beginning???

In recent past it has been observed that in most of the cases non-technical peoples are actually earning more than the white collar roles at the beginning of their career in different farms. Is it...

16 April 2019 5,664 19 View

Do you think your physical fitness can impact your research capabilities?

Being healthy is really important and I do believe staying physically fit can impact your research in a positive way due to the direct correlation of our mental physical wellness. Please share...

03 April 2019 4,669 86 View

Chitosan coated with alginate?

I am wondering if is it possible to firstly encapsulate bacteria with chitosan and then coat them within alginate-beads? Can anybody provide me with the procedure? Thank you

08 November 2018 6,625 3 View

Do I need to remove microbeads before putting CD31+ positively selected cells in culture?

I am isolating cells by CD31+ microbeads using magnet?

18 December 2015 9,805 3 View

Could anyone help with why the protein (Myc-DDK tagged) not expressing in HEK293T cells?

I am working on a protein which is encoded by 6.3kb of cDNA. The expected molecular weight of the protein is 236kDa. I have the commercially purchased cDNA clone of the same in a Myc-DDK backbone...

20 August 2014 2,528 5 View

Is there any software for predicting disulphide bonds in a protein?

I want to see the possible disulphide bond formations in a particular protein.

23 October 2013 2,572 18 View

What are the essential criteria for designing a probe for northern blot?

What should be the minimum probe length, the GC content and annealing temperature? Is a DNA or RNA probe more preferable? Is there any software to help design probes or can I just pick up a...

12 August 2013 8,113 6 View

What are the best possible softwares for ORF prediction?

Prediction softwares especially with respect to structure and function

01 October 2012 4,435 9 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Can anyone help me with the administration of the "Mind the Gap" Scale for satisfaction with transition to adult care?

We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...

28 February 2021 2,338 1 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View